Wildlife-vehicle collision (WVC) causes direct mortality and reduces population connectivity. Although the idea of WVC is well-characterized, its total impact on wildlife populations remains poorly understood. Here, we describe a method for estimating the impact of WVC at the US state scale, with a case study on California mule deer (Odocoileus hemionus). We used GenEst (USGS) (Dalthrop et al., 2018) to fit models, which incorporates rates of carcass observation, persistence, and effort, taken from our data collections systems, and search efficiency, from the literature.