Using observations of reported traffic incidents and carcasses the Road Ecology Center estimates the total annual cost of wildlife-vehicle conflict (WVC) in California to be at least $276 million, up 20% from the year before. This report includes maps of WVC hotspots, discusses impacts to wildlife and people from WVC, and ranks highways in each Caltrans District for financial cost of WVC (spoiler, I-280 in District 4 is the costliest). Projects to reduce WVC can be the most effective of any safety project, with effectiveness often >90%. In addition, only 1-2% of California’s transportation budget, including the new Senate Bill 1 funds, would be required to carry out these safety projects.